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| Id: | 8326
| | Autor: | Taylor, P. J; Salm, P; Lynch, S. V; Pillans, P. I
| | Título: | Simultaneous quantification of tacrolimus and sirolimus, in human blood, by high-performance liquid chromatography-tandem mass spectrometry^ien
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| | Fuente: | New York; Lippincott Williams & Wilkins; 2000. 608-12 p. ^btab, ^bgraf.
| | Resumen: | In this paper the authors present a validated method for the simultaneous analysis of tacrolimus and sirolimus in human blood by high-performance liquid chromatography-electrospray tandem mass spectrometry. Blood samples (500 microL) were prepared by C18 solid-phase extraction. Mass spectrometric detection was by selected reaction monitoring. The assay was linear for both compounds over the range 0.25-100 microg/L (r2 > 0.996, n = 7). At the limit of quantification (0.25 microg/L), for both sirolimus and tacrolimus, the interday imprecision was < 3 percent and the analytical recovery was between 97.0 percent and 102 percent , respectively. The interbatch and intrabatch coefficients of variation of the method for both analytes, at the three quality control concentrations (0.5, 20, and 80 microg/L), were < 16 percent and < 10 percent , respectively. The analytical recovery, at the three control concentrations, ranged from 99.2 percent to 104 percent of the nominal concentration. The mean absolute recovery (+/- standard deviation) of tacrolimus, sirolimus, and internal standard was 82 +/- 7 percent , 89 +/- 12 percent , and 77 +/- 8 percent , respectively (n = 12). In conclusion, the method presented can be used for simultaneous determination of tacrolimus and sirolimus and will aid in pharmacokinetic studies and therapeutic drug monitoring of these drugs. Furthermore, this method has economic benefits in the clinical setting where these drugs are coadministered. (AU)^ien.
| | Descriptores: | Espectrometría
Tacrolimus
Sirolimus
| | Límites: | Humanos
| | Localización: | PE14.1 |
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